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Record five-time Queen's Club champion Andy Murray opens his campaign against Australian Alex de Minaur, who beat the 36-year-old in Monte-Carlo earlier this season.
Former world No 1 Murray could meet Diego Schwartzman in the second round and third seed Taylor Fritz in the quarter-finals.
World No 2 Carlos Alcaraz will face a qualifier on his debut at the tournament and could face two-time defending champion Matteo Berrettini in the third round.
The top seed headlines the draw in London, where he will face Jiri Lehecka or Alejandro Davidovich Fokina in the second round if he wins his opening match.
Fourth seed Frances Tiafoe, who starts against Botic van de Zandschulp, is a potential semi-final opponent for Alcaraz.