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Defending champion Matteo Berrettini has withdrawn from the cinch Championships at Queen's Club due to an abdominal injury.
The 27-year-old Italian missed the clay-court season due to a stomach muscle tear and lost 6-1 6-2 to Lorenzo Sonego last week on his return to action in Stuttgart.
Berrettini joined an elite group of players last summer when beating Filip Krajinovic in the final to become one of eight players in the open era to successfully retain the Queen's Club crown.
The world No 21 was runner-up to Novak Djokovic at Wimbledon in 2021 – he withdrew last year after testing positive for Covid - and it remains to be seen if the Italian will be fit enough to compete at the All England Club next month.