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Barcelona wasted a golden opportunity to extend their lead at the top of LaLiga as they were shocked 1-0 at Almeria on Sunday after a first-half strike by El Bilal Toure.
But they remain top of the league standings on 59 points, seven ahead second-placed Real Madrid who were held to a 1-1 draw at home by Atletico Madrid on Saturday.
Almeria are 15th on 25 points, two clear of the relegation zone.
Almeria were the better side in the first half and scored after 24 minutes when Luis Suarez sent a perfect long pass to Toure who was ghosting behind Barca's defence.
The Malian striker ran towards the area and hit a thunderous strike in off the crossbar.
Barcelona had not lost in 13 league games, with only one draw and 12 wins going back to their last defeat at Madrid in October.
Bayern Munich top-scorer Jamal Musiala struck again on his birthday to help his team reclaim first place in the Bundesliga with a 3-0 win over surprise challengers Union Berlin.
After goals from Eric Maxim Choupo-Moting and Kingsley Coman, Musiala marked his 20th birthday with his 11th league goal of the season just before the break for Bayern to deal Union their first defeat in any competition since November.
The 10-time defending champions reclaimed the lead on goal difference from Borussia Dortmund, who have closed the gap by winning every game they have
Union dropped three points behind the big two in third place.
Earlier, Sardar Azmoun scored for Bayer Leverkusen to salvage a 1-1 draw at Freiburg, but the team's patchy form leaves European qualification increasingly at risk.
Leverkusen have won three of their seven league games so far this year, and Sunday's draw dropped the team down to 11th.
Freiburg dropped to fifth, one point behind Leipzig in the last place for Champions League qualification.